Select Update Driver. Cell Cytotoxicity assay procedureMolecular Devices. Molecular Devices Software. The plate reader optics are. It features a modular design that allows users to add or change detection cartridges according to their needs. We therefore gathered salient. Multi-mode Microplate Readers. Beyond the hardware, a microplate reader’s software also impacts performance and flexibility. Changing Plate Hold-Down Devices 123 Cell Reservoir 125 Chapter 7: Calibration and Signal Test 127. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. #4TI-0655) Microplates. Unplug the power cable from the back of the instrument. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Flexibility to read a variety of plate formats. VIS absorbance detection. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Dual monochromators increase performance and assay flexibility. 7°C and the bottom to 36. PURPOSE. Plate reader settings used for data acquisition of bacterial growth curve. 0 × 107) RLU 0. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Perform plate-based assays on a large number of plates without having to manually feed them into your microplate washer and reader. Complete plate reader solution for all your research needs. We would like to show you a description here but the site won’t allow us. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great. To update the driver. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Absorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. Compact designs. Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. Vmax® plate reader specifications. 9000-0756. The plate was placed on a shaker for 30 seconds and then incubated for 3 hours at 37°C, 5% CO 2. +44-118-944-8000. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:[The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. 1. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Feb 22, 2023Fast and compact absorbance microplate readers for a wide range of assays without the use of filtersSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. The plate reader can measure multiple readouts, including UV and. Search. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. W×D×H. These robust readers have been placed in labs from. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. ** Some compatible filter sets are available for purchase from Life. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. 10,700. No accessories are needed and SoftMax® Pro Software. SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models. Find our technical support information along with contact details. Molecular Devices i3x. Molecular Devices also offers Digital IQ/OQ/PM. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 1 up to 3600 sec. Integrated to. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). One plate carrier adapter is provided with the instrument. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Double-click the unknown device with the yellow warning icon. 9. Testing can be done in the user’s lab on their own schedule. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. SpectraMax Microplate Readers. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Figure 1. Click on each for more information. Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. 9. This can increase throughput and efficiency, thus saving time and space in the lab. 00. , Sunnyvale, CA 94089, USA, Jin_Liao@moldev. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Figure 1. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Molecular Devices' microplate readers. In this unique new five-part weekly webinar series, running every Thursday throughout October 2020, we’ll introduce you to various aspects of microplate reader technology in an educational and entertaining way! Starting with some basic concepts,. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Multimode microplate readers deliver both performance and economy. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. To update the driver. ~ Bio-Tek ELx808 Microplate Reader $2,795. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. #SpectraMax L Config) Methods. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented. 1 sec. NOT FOR USE IN DIAGNOSTIC PROCEDURES. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. Molecular Devices' microplate readers. (a) Chemical structures of 1 (HADP) and its analogues 2–4. SoftMax Pro 7. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. HTRF-compatible SpectraMax Multi-Mode Readers. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Reader Documentation Version No. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. SoftMax Pro Software. #4TI-0214) UltraVision Plate 96 (Brooks Life. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. , prior to compound addition) from the cells. SpectraMax® Microplate Readers and SoftMax® Pro Software. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. The plate was incubated at room temperature for 50 minutes or 2. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. V. 1). It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Fluorescence Microplate Reader Comparison Chart. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. 1. 747. 76358-624. together with a Molecular Devices plate. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Select the Driver tab. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Read More. Contact our support team with your questions regarding Molecular Devices products and applications. StakMax Handling Stacker Base Unit. and fluorescence microplate readers—. Built-in Help function answers your most frequently asked questions. 6% for the on-board optics (monochromators). 0 software Download page. The Spectratest® Abs1 plate, which is used for validationof Molecular Devices monochromator based readers, cannot be used to validate the Emax/Vmax readers. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Data acquisition and analysis. F3/F5 Multi Mode Microplate Readers. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. HTRF-compatible SpectraMax Multi-Mode Readers. 0 software Download page. 2, 2022 – Molecular Devices announced today the launch of the ValitaCell IgG titer assay, ValitaTiter, available for global distribution. It consists of a SpectraMax® Microplate Reader, Peak Robotics S-LAB Plate Handler with the S-LAB software, and the AquaMax® 4000 Microplate Washer. Luminescence detection typically offers greater sensitivity than absorbance or fluorescence detection. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. (a) Chemical structures of 1 (HADP) and its analogues 2–4. or washer including mechanical, electrical, and optical components to verify operating functions are in accordance to the Molecular Devices product operational specifications. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 9. 1. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. This document is provided to customers who have purchased Molecular Devices, Inc. 5 µL of each sample was pipetted into a 96-well. Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability making it easy to evolve your system alongside your research. Microplate Stacker and Washer. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. These models can no longer be repaired. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. Explore our fully-integrated,. #Flex3) Methods. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Molecular Devices Software. Some plate readers handle label-free cell-based assays, such as PerkinElmer’s EnSpire® multimode reader, which in addition to absorbance, luminescence, fluorescence and AlphaLISA also supports Corning’s Epic® technology. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Select Start>Control panel>Device Manager. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Select the Driver tab and click Update Driver. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. FlexStation® 3 Multi-Mode Microplate Reader. SpectraMax readers support page; Analyst and FlexStation reader support page; Plate handlers and washers support page; We are continuously adding material to the knowledge base. 9. 8 foot) 4400-0002 Power cord, 1 meter (3. Evaluation of BioTek's Synergy H4 Plate Reader for Fluorescent Detection. By measuring optical pathlength, it eliminates the last. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Tests every subsystem of the reader . The adapter is required for optimum performa nce with standard 96-well and 384-. This can increase throughput and efficiency, thus saving time and space in the lab. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Complete plate reader solution for all your research needs. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. Uncontrolled Copy for Training and Reference Purposes Only . The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. At the beginning of a data run, perform a ìsignal testî to check the basal fluorescence signal (i. ABS Plus can run both cuvette-based and microplate reader applications on the same instrument. to that of dedicated single-mode readers. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. ® Plate chamber design of the SpectraMax category 1 microplate readers. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. Supplier: Molecular Devices. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 9000-0756. Instrument settings can be saved as a protocol file. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. How to import latest protocol revisions into SoftMax Pro Protocol Library; Protocols for the ABS2 Validation Plate. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The CellTiter-Glo assay from Promega uses luciferase. 211, and 820 and EudraLex Volume 4 and Annex 11 and 15, and how they apply to the implementation of Molecular Devices microplate readers and SoftMax Pro. ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. . D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Supplier: Molecular Devices. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. System setup and calibration are controlled from within SoftMax Pro Software. An end-to-end solution for high-content imaging and analysis. 0200-6263. Unparalleled performance on a personalized platform. 1 up to 3600 sec. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Shop now. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. The reader measures both flat and round 96-well microplates and. In addition, the iD5 reader measures TRF and FP and. Cellular Imaging Systems. Endpoint, kinetic, spectrum. Clone. View SoftMax Pro GxP. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. . ABS Test results. Tristar 3 Multimode Microplate Reader. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. ” So, acquisition and analysis come from one software system. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. Every step is optimized to simplify analysis and reporting to support our microplate readers. 1. The calculated LOQ values were 0. A standard spectrophotometer measures the absorbance of one sample at a time. Eight filter options provide simplified assay setup. *Purchase is subject to Molecular Devices standard terms and conditions of sale. 5. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. 2. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. 7°C and the bottom to 36. Instrument settings can be saved as a protocol file. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Microplate readers and controlling software enable walk away data analysis. book Page 1 Tuesday, July 6, 2010 2:08 PM SpectraMax Microplate Readers. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W. SpectraMax Paradigm Multi-Mode Safety Guide From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?Sunnyvale, California -- October 5, 2017. Plus 384. The adapter is required for optimum performa nce with standard 96-well and 384-. Supersedes 1. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. SoftMax Pro Software. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableThe SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. 1. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. Fortunately, Molecular Devices’ SpectraMax i3x and iD5 readers offer the best of both worlds. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Published Dec 16, 2020 | Updated Dec 16, 2020. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. 1. Step 2: Launch SoftMax Pro Software. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. 0A-Sunnyvale, CA-Made in USAWe specialize in many of the Molecular Devices microplate readers, washers, and stackers including the SpectraMax®, VersaMax™, Gemini™, FlexStation™, AquaMax®, and StackMax® lines. However, when a specific probe is not available, or it causes off-target effects on the. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofRead plate standard and mix. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Versa Max. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. absorbance readers. If the computer is connected to the Internet: Select Search Automatically for updated driver software. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. Also refer to the Multimode Reader Comparison Chart Features. Ensure data accuracy of your reader. in steps of 0. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 304 signal level saturation. S. The StakMax system integrated with Molecular Devices microplate readers and AquaMax washers provides the walk-away automation for up to 50 microplates. Plate pre-read measurements were made in a Costar UV plate. View SoftMax Pro GxP. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Introduction. to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and. Step 4: Incubate at room temperature for 1 hour, protected from light. Molecular Devices microplate readers. Monday to Friday 8am - 5pm GMT. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader. Wait 20 seconds. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Microplate Reader Comparison Charts. SoftMax Pro. Fluorescence Microplate Reader Comparison Chart. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. SpectraDrop Micro-Volume Microplate (Molecular Devices cat. In . The touchscreen interface provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Eliminate time-dependent substrate addition steps. Absorbance Plate Reader Comparison Chart. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Search. Turn on Plate Reader and wait for it to complete its start-up routine. SoftMax Pro GxP Software. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Flexibility to read a variety of plate formats. The new assay will complement Molecular Devices’ workflow solution for cell line development by offering a 10-minute titer assay that can be run on the company’s SpectraMax series of microplate. Shaking. Turn the reader on and secure the USB cable. It may be named "Pothos". Increase productivity with patented microplate reader technologies. Molecular Devices' microplate readers. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. 1. The advantage of using labeled probes is detection specificity. Microplate Readers. SoftMax® Pro Software Version 5 for Mac and Windows User Guide 5000938 B June 2010 SoftMaxPro_UserGuide_5000938. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Superior opticsThe top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. FLIPR Tetra fluorometric imaging plate reader (Molecular Devices, Inc) with EMCCD Camera and 440–480 nm and 515–575 nm emission filters; Methods Cell Preparation. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Fig 2: Enzymatic response of synthetic compounds. Learn more about their features and specifications in. Summary of Contents for Molecular Devices SpectraMax M2. NORTH AMERICA. Molecular Devices Software. Step 1: Turn on your microplate reader. #iD3) Methods. For researchers working in GLP or GMP laboratories, achieve full GxP compliance with our turnkey solution:. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 58% for the detection module and 2. The luminescent reaction must be very intense for a reliable reading. 7°C (gradient heating design). The percent The percent (4) Asiaticoside reverses M2 phenotype macrophage polarization-evoked osteosarcoma cell malignant behaviour by TRAF6/NF-κB inhibition Pharmaceutical. Page 17 Chapter 2: Setting Up the Instrument 4. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Urban myths of microplate readers. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 190. M5. The Synergy H4 is a convenient benchtop microplate reader that offers robust. 1. Recertification service. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. It then uses the pre-defined settings for the specified instrument to build a protocol. a single plate read using any standard cuvette, or 96- or 384-well microplate. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 과 같은 Parameter를 검사하여 평가할 수 있습니다. ¨Automated microplate readers reduce manual manipulation and ensure consistent operation. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Details. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Microplate Readers. Fig 2: Enzymatic response of synthetic compounds. 1. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Instrument settings can be saved as a protocol file. The whole plate can be detect in a single run with ease and flexible.